T6414
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 | Trypsin Inhibitor (Kunitz) (soybean) |
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T6414
Sigma
Trypsin inhibitor from Glycine max (soybean)
1 ×, solution, sterile-filtered, cell culture tested
| Synonym: | SBTI |
| CAS Number: | 9035-81-8 |
| MDL number: | MFCD00082103 |
Description
| Application | Optimized for passage of endothelial cell cultures. |
| Other Notes | Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum. |
| View more information on Trypsin Inhibitor. | |
| Preparation Note | Prepared in Dulbecco's Phosphate Buffered Saline without calcium and magnesium. |
| Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color. | |
| Biochem/physiol Actions | Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108. |
Properties
| sterility | sterile-filtered |
| form | solution |
| concentration | 1 × |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 2 |
